Online Inquiry
DNMT1 (Δexons3-5/Δexons3-5); DNMT3B (-/-) retains CH3 Cell Line
SPL-01160
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| DNMT1 (Δexons3-5/Δexons3-5); DNMT3B (-/-) retains CH3 |
| Target Information | |
|---|---|
| Target Name | DNMT1 |
| Gene Abbr. | DNMT1 |
| Gene ID | 1786 |
| Full Name | DNA methyltransferase 1 |
| Alias | ADCADN, AIM, CXXC9, DNMT, HSN1E |
| Species | Human |
| Genomic Locus | 19p13.2;20q11.2 |
| Introduction | This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]. |
| Product Details | |
|---|---|
| Cell Line Model | HCT116 |
| Genotype | DNMT1 (Δexons3-5/Δexons3-5); DNMT3B (-/-) retains CH3 |
| Description | DNMT1 (Δexons3-5/Δexons3-5); DNMT3B (-/-) retains CH3 |
| Parental ATCC ID | CCL-247 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 7,10; D13S317: 10,12; D16S539: 11,13; D5S818: 10,11; D7S820: 11,12; THO1: 8,9; TPOX: 8,9; vWA: 17,22; ATCC |
| Disease | Colorectal carcinoma |
| Tissue | Colon |
| Gender | Male |
| Key Endogenous Mutations | CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1 |
| CVT Selection Condition | G418 resistant - 0.4 mg/mL; Hygromycin resistant - 0.1 mg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20. |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.