Dendrimer-Hyaluronic Acid Conjugation Service

Dendrimers modified with hyaluronic acid not only allow cell-specific gene delivery, but also overcome drug resistance and effectively kill cancer cells. As a trusted and fast-growing biotechnology company, CD BioSciences offers the most comprehensive and effective solutions for dendrimer engineering services. We are committed to helping our customers solve all the problems encountered in the dendrimer-hyaluronic acid conjugation process to advance their applications in the biomedical field.

Introduction into Hyaluronic Acid

Hyaluronic acid (abbreviated as HA), which also becomes hyaluronan, is widely distributed in connective, epithelial and neural tissues and is an anionic, nonsulfated glycosaminoglycan. Hyaluronic acid is a hydrophilic anionic polymer, usually in the form of extracellular matrix (ECM), consisting of repeating disaccharides of N-acetyl-glucosamine and glucuronic acid that is connected by β-linkages, and is involved in cell biology, such as cell migration, proliferation, differentiation, adhesion, and gene expression. In addition to its excellent biocompatibility and biodegradability, hyaluronic acid has been applied to target overexpressed receptors in cancer cells, such as the CD44 receptor. CD44 is a highly specific single-stranded surface glycoprotein with a high degree of specificity encoded by a single gene. CD44 is relatively quiescent on the surface of many normal cells, while it is overexpressed and highly activated on the surface of tumor cells. CD44 is the main receptor for HA and is highly expressed on the surface of several tumor cells such as MCF-7 and MDAMB-231, thus HA can increase intracellular drug concentrations in tumor tissues.

Dendrimer-Hyaluronic Acid Conjugation for siRNA Delivery

The biggest obstacle for siRNA as a safe and effective gene therapy drug is that free siRNA is susceptible to enzymatic degradation in vivo. Therefore, stable, safe, and effective delivery methods are essential for siRNA-based gene therapy. The vectors used for gene delivery include viral and non-viral vectors, although viral vectors exhibit high transfection efficiency, but their clinical application is hampered by low safety. Non-viral nanocarriers such as cationic polymers, such as poly(amidoamine) dendrimers (PAMAM), polyethyleneimine (PEI) and poly(L-lysine) (PLL) are widely used for siRNA delivery. As a nanoparticle, dendrimers, such as PAMAM, have well-defined size and shape and show great promise for applications in delivering siRNA.

When HA is conjugated to dendrimers as a ligand, it can be used as a delivery vehicle for gene drug siRNA to target delivery to CD44 overexpressing tumor cells. In addition, dendrimer micelles (MDMs) modified by hyaluronic acid can not only form a stable complex with siRNA to maintain siRNA integrity and prevent RNase degradation, but also alleviate the toxicity of cationic charge and improve the targeting to cancer cells. When the dendrimer is modified by HA it can also deliver both doxorubicin and siRNA to overcome drug resistance and effectively kill cancer cells.

Schematic illustration of the hyaluronic acid-modified mixed dendrimer micelles for co-delivery of siMDR-1 and doxorubicin base. (Zhang X, et al., 2020)

Our Services

With sophisticated facilities and experienced scientists, CD BioSciences provides dendrimer engineering services to meet market needs for customers worldwide. We are committed to cost-effective dendrimer-hyaluronic acid conjugation services and to increasing the availability of dendrimers in biomedicine by breaking the limitations of traditional gene delivery vectors. Our expertise can help our customers overcome all the difficulties in dendrimer modification and application.

Service Process

Want to Learn More?

As a trusted CRO, CD BioSciences provides the most comprehensive and effective solutions for dendrimer engineering services to advance dendrimer products development and applications in the biomedical field. We deliver rigorously quality-tested results with fast turnaround times to ensure your satisfaction. If you are interested in our services or have any additional questions, please feel free to contact us and we look forward to working with you on attractive projects.

Reference

1. Zhang X, et al., Palmerston Mendes L, Sarisozen C, Mao S, Torchilin VP. Charge reversible hyaluronic acid-modified dendrimer-based nanoparticles for siMDR-1 and doxorubicin co-delivery. Eur J Pharm Biopharm. 2020, 154: 43-49.