Cat#: CCR-0043
Azide-PEG3-biotin conjugate
Synonyms (s): N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]hexahydro-2-oxo-(3aS,4S,6aR)- 1H-thieno[3,4-d ]imidazole-4-pentanamide, Biotin-PEG3-azide
Empirical Formula (Hill Notation): C18H32N6O5S
Size: 10 mg; 25 mg
Product Introduction
| Molecular Weight | 444.55 |
| CAS Number | 875770-34-6 |
| MDL Number | MFCD20134145 |
| PubChem Substance ID | 329766598 |
| Form | Solid |
| Reaction Suitability | Reaction type: click chemistry |
| Smiles String | O=C1N[C@](CS[C@H]2CCCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)([H])[C@]2([H])N1 |
| InChI | 1S/C18H32N6O5S/c19-24-21-6-8-28-10-12-29-11-9-27-7-5-20-16(25)4-2-1-3-15-17-14(13-30-15)22-18(26)23-17/h14-15,17H,1-13H2,(H,20,25)(H2,22,23,26)/t14-,15-,17-/m1/s1 |
| InChI key | ZWFOOMQCYIGZBE-BFYDXBDKSA-N |
| Storage Temp. | -20 °C |
Application
Azide-PEG3-biotin conjugate has been used for the detection of a cellular alkyne cholesterol analog via click chemistry using fluorescence microscopy after incubation with a fluorescent streptavidin-conjugate. It has also been used to prepare a novel multifunctional benzophenone linker that can be used for pull-down assays, photoaffinity labeling and double-click stapling techniques.
Biotinylation reagent for labeling alkyne containing molecules or biomolecules using either copper-catalyzed 1,3 dipolar cycloaddition click chemistry or copper-free click chemistry with cyclooctyne derivatives. The azide group reacts with alkynes to form a stable triazole linkage facilitating the introduction of biotin into your alkyne modified system of interest. This reaction is bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.
Safety Information
| Storage Class Code | 11 - Combustible Solids |
| WGK | WGK 3 |
For research use only. Not for clinical use.
