Cat#: CLP-0062
APN-Azide
Synonyms (s): 3-(4-Azidophenyl)propiolonitrile, APN-N3
Empirical Formula (Hill Notation): C9H4N4
Size: 50 mg
Product Introduction
| Molecular Weight | 168.15 |
| CAS Number | 1643841-88-6 |
| PubChem Substance ID | 329769021 |
| Assay | ≥97.0% (NT) |
| Form | Powder |
| Reaction Suitability | Reagent type: Cross-linking reagent |
| Smiles String | N#CC#CC1=CC=C(N=[N+]=[N-])C=C1 |
| InChI | 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H |
| InChI key | BDLXITYEYYHQNK-UHFFFAOYSA-N |
| Storage Temp. | 2-8 °C |
Application
APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).3. Incubate at room temperature for 2 hours.4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.*Note: avoid thiol-containing buffers.
Safety Information
| Signal Word | Danger |
| Target Organs | Respiratory system |
| Hazard Classifications | Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3 |
| Storage Class Code | 4.1B - Flammable solid hazardous materials |
| WGK | WGK 3 |
For research use only. Not for clinical use.
