5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

Synonyms (s): 5-DBCO-PEG4-dUTP

Empirical Formula (Hill Notation): C₄₂H₅₄N₅O₂₁P₃

Size: 50 μL

Product Introduction

Molecular Weight	1057.82
NACRES	NA.22
PubChem Substance ID	329769180
Assay	>95% (HPLC)
Form	Solution
Reaction Suitability	Reaction type: click chemistry
Smiles String	O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
Storage Temp.	-20 °C

Application

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

Safety Information

Storage Class Code	12 - Non Combustible Liquids
WGK	WGK 2

For research use only. Not for clinical use.