γ-(2-Azidoethyl)-ATP sodium salt solution

Synonyms (s): γ-(2-Azidoethyl)-adenosine-5’-triphosphate sodium salt solution

Empirical Formula (Hill Notation): C₁₂H₁₉N₈O₁₃P₃ · xNa⁺

Size: 100 μL

Product Introduction

Molecular Weight	576.25 (free acid basis)
NACRES	NA.22
PubChem Substance ID	329769106
Assay	≥95% (HPLC)
Form	Aqueous solution (pH 7.5)
Reaction Suitability	Reaction type: click chemistry
Smiles String	O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2)O[C@@H]1COP(OP(OP(OCCN=[N+]=[N-])(O)=O)(O)=O)(O)=O.C
Storage Temp.	-20 °C

Application

Lee, et al., reported a non-radioactive version of in vitro phosphorylation where γ-[2-Azidoethyl]-ATP (compound 8) was successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can be labeled with alkynes of biotin or fluorescent dyes using Cu(I)- catalyzed click chemistry or DBCO-containing biotin or fluorescent dyes using Cu(I)-free click chemistry.

Safety Information

Storage Class Code	12 - Non Combustible Liquids
WGK	WGK 2

For research use only. Not for clinical use.