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D4-GDI/RhoGDI2 (cleavage specific) Antibody
SPA-02773
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | D4-GDI/RhoGDI2 - (cleavage specific) |
| Gene Abbr. | ARHGDIB |
| Gene ID | 397 |
| Full Name | Rho GDP dissociation inhibitor beta |
| Alias | D4, GDIA2, GDID4, LYGDI, Ly-GDI |
| Introduction | D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | Available upon request. |
| Isotype | IgG2B Kappa |
| Immunogen | A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen. |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| Dilutions | Western Blot (2 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells); Immunohistochemistry (1:10-1:500); Immunoprecipitation (1-2 µg/mL) |
| Reactivity | Human, Mouse |
| Specificity | In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS containing 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.