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Cytochrome c Antibody
SPA-02672
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Cytochrome c |
| Gene Abbr. | CYCS |
| Gene ID | 54205 |
| Full Name | cytochrome c, somatic |
| Alias | CYC, HCS, THC4 |
| Introduction | Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form. This event further triggers caspase-3 activation and eventually leads to apoptosis. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | CTC05 |
| Isotype | IgG2B Kappa |
| Immunogen | Recombinant cytochrome c protein. |
| Usage | |
|---|---|
| Application | WB, FC, IF, IHC |
| Dilutions | Western Blot (0.5-1.0 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells in 0.1 mL) |
| MW(KDa) | 14 |
| Reactivity | Human, Mouse, Rat, Am, Avian, Canine, D. melanogaster, Equine |
| Specificity | Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 10 mM PBS with 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.