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Cyclin E1 Sandwich ELISA Kit
SPK-00241
| Size | Price |
| 1 X 96T | Online Inquiry |
| 5 X 96T | Online Inquiry |
| 10 X 96T | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Cyclin E1 |
| Gene Abbr. | CCNE1 |
| Gene ID | 898 |
| Full Name | cyclin E1 |
| Alias | CCNE, pCCNE1 |
| Introduction | Cyclin E1 and cyclin E2 can associate with and activate CDK2. Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2. Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis. Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation. Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3. |
| Product Details | |
|---|---|
| Measurement | Sandwich |
| Usage | |
|---|---|
| Application | ELISA |
| Reactivity | |
| Assay Principle | quantitative |
| Sensitivity | 6.25 pg/mL |
| Detection Range | 25 pg/mL - 1600 pg/mL |
| Sample Volume | 50-100μL |
| Assay Time | 1-5h |
| Detection Wavelength | 450 nm |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8%. Inter-assay Precision (Precision between assays): CV%<10%. |
| Storage & Handling | |
|---|---|
| Storage Temp. | Store at 2-8°C. Please refer to protocol. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.