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CXCL14/BRAK Antibody
SPA-02497
| Size | Price |
| 25 µg | Online Inquiry |
| 500 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CXCL14 |
| Gene Abbr. | CXCL14 |
| Gene ID | 9547 |
| Full Name | C-X-C motif chemokine ligand 14 |
| Alias | BMAC, BRAK, KEC, KS1, MIP-2g |
| Introduction | CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP-2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703. Cao, X. et al. (2000) J. Immunol. 165:2588. Kurth, I. et al. (2001) J. Exp. Med. 194:855. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 131120 |
| Isotype | IgG2A |
| Immunogen | E. coli-derived recombinant human CXCL14/BRAK. Leu23-Tyr99. O95715. |
| Usage | |
|---|---|
| Application | WB, ELISA |
| Dilutions | Western Blot (1 µg/mL); ELISA Capture (2-8 µg/mL); ELISA Detection (0.5-2.0 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human CXCL14/BRAK in ELISAs and Western Blots. In direct ELISAs, shows approximately 80% cross-reactivity with recombinant mouse (rm) CXCL14/BRAK and no cross-reactivity with recombinant human (rh)GRO alpha, rhGRO beta, rhGRO gamma,rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.