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CXCL13/BLC/BCA-1 Antibody
SPA-02491
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CXCL13 |
| Gene Abbr. | CXCL13 |
| Gene ID | 10563 |
| Full Name | C-X-C motif chemokine ligand 13 |
| Alias | ANGIE, ANGIE2, BCA-1, BCA1, BLC |
| Introduction | CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas. Gunn, M.D. et al. (1998) Nature, 391:799. Legler, D.F. et al. (1998) J. Exp. Med. 187:655. Forster, R. et al. (1996) Cell 87:1037. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 53610 |
| Isotype | IgG1 |
| Immunogen | E. coli-derived recombinant human CXCL13/BLC/BCA-1. Val23-Arg94. O43927. |
| Usage | |
|---|---|
| Application | WB, ELISA, FC |
| Dilutions | Western Blot (1 µg/mL); ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL); Flow Cytometry (2.5 µg/10^6 cells); Neutralization (0.3-0.9 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human CXCL13/BLC/BCA-1 in ELISAs and Western Blots. In ELISAs, no cross-reactivity with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL‑8, rhCXCL10, rhCXCL9, rhCXCL12/SDF‑1 alpha, or rhCXCL12/SDF‑1 beta is observed. In Western Blots, no cross‑reactivity with recombinant mouse CXCL13/BLC/BCA-1 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.