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CTNNB1 (T41A/+/+) Cell Line
SPL-01080
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| CTNNB1 (T41A/+/+) |
| Target Information | |
|---|---|
| Target Name | β-Catenin |
| Gene Abbr. | CTNNB1 |
| Gene ID | 1499 |
| Full Name | catenin beta 1 |
| Alias | CTNNB, EVR7, MRD19, NEDSDV, armadillo |
| Species | Human |
| Genomic Locus | 3p21 |
| Introduction | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]. |
| Product Details | |
|---|---|
| Cell Line Model | MCF10A |
| Genotype | CTNNB1 (T41A/+/+) |
| Description | CTNNB1 (T41A/+/+) |
| Parental ATCC ID | CRL-10317 |
| STR Profile | Amelogenin: X; CSF1PO: 10,12; D13S317: 8,9; D16S539: 11,12; D5S818: 10,13; D7S820: 10,11; THO1: 8,9.3; TPOX: 9,11; vWA: 15,17; ATCC |
| Disease | Epithelial |
| Tissue | Breast |
| Gender | Female |
| Key Endogenous Mutations | none listed |
| CVT Selection Condition | No antibiotic resistance |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 80-90% confluency, approximately 1:6-1:10. |
| Culture Medium | DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 5% horse serum, 10 µg/mL insulin, 20 ng/mL hEGF, 0.5 µg/mL hydrocortisone, 0.1 µg/mL cholera toxin. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 80-90% confluency, approximately 1:6-1:10. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.