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Cool2/αPix Antibody
SPA-02295
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Cool2/αPix |
| Gene Abbr. | ARHGEF6 |
| Gene ID | 9459 |
| Full Name | Rac/Cdc42 guanine nucleotide exchange factor 6 |
| Alias | COOL2, Cool-2, MRX46, PIXA, alpha-PIX |
| Introduction | Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/αPix and cool1/βPix, the latter having two splice variants that vary in their carboxy termini. Cool2/αPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain.X-chromosomal genes mutated in nonspecific mental retardation (MRX) comprise a family of genes, including the gene encoding Cool2/αPix, thought to be involved in mental retardation.Cool2/αPix interacts with β-parvin/affixin, a protein involved in integrin signaling and may act downstream of integrin-linked kinase (ILK) to regulate actin reorganization and cell spreading.When Cool2αPix exists as a dimer, it functions as a Rac-specific GEF, whereas the monomeric protein acts as a GEF for both Rac and Cdc42. Regulation of Cool2/αPix dimerization, and therefore its specificity, occurs at least in part through p21 activated kinase (PAK) in response to extracellular signaling. Further, binding of Cdc42 enhances the Rac GEF activity of the Cool2/αPix dimer. Activated Rac in turn inhibits Cool2/αPix Rac GEF activity. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C23D2 |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of human Cool2/αPix. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Cool2/αPix. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 87 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total Cool2/αPix protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.