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CLK1 Knockout Cell Line
SPL-00994
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 5bp insertion |
| Target Information | |
|---|---|
| Target Name | CLK1 |
| Gene Abbr. | CLK1 |
| Gene ID | 1195 |
| Full Name | CDC like kinase 1 |
| Alias | CLK, CLK/STY, STY |
| Species | Human |
| Genomic Locus | chr2:200861321 |
| Transcript | NM_004071 |
| WT Expression Level | 28.28 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 5bp insertion in a coding exon of CLK1. |
| Description | 5bp insertion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | TGCTACTATGGTTCTGATAC |
| PCR Primer |
Forward: TCCCTGTTCCACATGGGATATAAAA Reverse: CTCTAGCCATTATTTGGAAAGCAGG |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.