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Cleaved-PARP Antibody
SPA-08599
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PARP |
| Gene Abbr. | PARP1 |
| Gene ID | 142 |
| Full Name | poly(ADP-ribose) polymerase 1 |
| Alias | ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP |
| Introduction | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 194C1439 |
| Isotype | IgG2B Kappa |
| Immunogen | This antibody was developed by immunizing mice with a synthetic peptide containing amino acids near 214/215-cleavage site of human PARP. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1-2 µg/mL); Immunofluorescence (2-5 µg/mL) |
| MW(KDa) | 89 |
| Reactivity | Human, Mouse, Rat |
| Specificity | This monoclonal antibody specifically detects a 89-kDa cleavage fragment containing the catalytic and auto modification domain. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.