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Cleaved Caspase-3 (Asp175) Antibody
SPA-01398
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Caspase-3 |
| Gene Abbr. | CASP3 |
| Gene ID | 836 |
| Full Name | caspase 3 |
| Alias | CPP32, CPP32B, SCA-1 |
| Introduction | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | Asp175 |
| Isotype | IgG |
| Immunogen | KLH-conjugated human Caspase-3 synthetic peptide. CRGTELDCGIETD. U26943. |
| Usage | |
|---|---|
| Application | WB, IHC, FC, IF |
| Dilutions | Western Blot (0.5 µg/mL); Immunohistochemistry (0.3-25 µg/mL); Immunofluorescence (8-25 µg/mL); Flow Cytometry (0.25 µg/10^6 cells) |
| MW(KDa) | 17, 19 |
| Reactivity | Human, Mouse |
| Specificity | Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.