CHEK2 cDNA ORF Clone, Human, untagged - CD BioSciences

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CHEK2 cDNA ORF Clone, Human, untagged

CHEK2 cDNA ORF Clone, Human, untagged

SPD-03343

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Description
Full length Clone DNA of Human CHK2 checkpoint homolog (S. pombe).
Target Information
Species Human
Target Name Chk2
Gene Abbr. CHEK2
Gene ID 11200
Full Name checkpoint kinase 2
Alias CDS1, CHK2, HuCds1, LFS2, PP1425
Introduction Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain.
Product Details
Description Full length Clone DNA of Human CHK2 checkpoint homolog (S. pombe).
NCBI Ref Seq NM_007194.3
RefSeq ORF Size 1632 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-untagged
Promoter Enhanced CMV promoter
Restriction Sites KpnI + XbaI (6.1kb + 1.63kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Ampicillin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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