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CELSR2 Antibody
SPA-02142
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CELSR2 |
| Gene Abbr. | CELSR2 |
| Gene ID | 1952 |
| Full Name | cadherin EGF LAG seven-pass G-type receptor 2 |
| Alias | ADGRC2, CDHF10, EGFL2, Flamingo1, MEGF3 |
| Introduction | CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant human CELSR2. Cys51-Phe231. Q9HCU4. |
| Usage | |
|---|---|
| Application | WB, FC, IHC |
| Dilutions | Western Blot (1 µg/mL); Flow Cytometry (2.5 µg/10^6 cells); Immunohistochemistry (5-15 µg/mL) |
| Reactivity | Human, Mouse |
| Specificity | Detects human CELSR2 in direct ELISAs and Western Blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.