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CDK8 Antibody
SPA-02140
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CDK8 |
| Gene Abbr. | CDK8 |
| Gene ID | 1024 |
| Full Name | cyclin dependent kinase 8 |
| Alias | IDDHBA, K35 |
| Introduction | The mammalian Mediator Complex is a multi-subunit protein complex that couples specific transcriptional regulators to RNA polymerase II (Pol II) and the basal transcription machinery. Interactions between distinct Mediator subunits and transcription factors allow for specific gene regulation.Mediator complex interactions control various biological processes, including insulin signaling NF-κB-dependent signaling stem cell pluripotency and self renewal, and proliferation of colon cancer cells.CDK8/Cyclin C, along with Med12 and Med13, constitute a subcomplex within the Mediator Complex thought to act as a molecular switch, inhibiting Pol II recruitment and transcription initiation. Expression of CDK8 abrogates E2F-1-dependent inhibition of β-catenin activity in colon cancer cells. High levels of CDK8 coincide with high β-catenin-dependent transcription in colon cancer cells, and their proliferation can be inhibited by suppressing CDK8 expression.CDK8 can phosphorylate Ser727 on STAT1, which reduces natural killer (NK) cell toxicity. As such, inhibitors are being pursued as potential therapeutics to enhance NK cell activity and combat a variety of cancer types. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gly398 of human CDK8. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly398 of human CDK8. Antibodies are purified using protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 53 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total CDK8 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.