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CD45RO Antibody
SPA-02055
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CD45RO |
| Gene Abbr. | PTPRC |
| Gene ID | 5788 |
| Full Name | protein tyrosine phosphatase receptor type C |
| Alias | B220, CD45, CD45R, GP180, L-CA |
| Introduction | The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression.Several isoforms of CD45 are generated through alternative splicing in a cell type-specific and activation state-specific manner. Memory T cells are positive for CD45RO, while naive T cells are negative for CD45RO. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | Available upon request. |
| Immunogen | The IL-2-dependent T cell line, CA1 (5). |
| Source/Purification | Antibody is produced by immunizing animals with the IL-2-dependent T cell line, CA1. |
| Usage | |
|---|---|
| Application | IHC |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | CD45RO Mouse mAb (IHC Specific) recognizes endogenous levels of total CD45RO protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.