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CD40/TNFRSF5 Antibody
SPA-02033
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CD40 |
| Gene Abbr. | CD40 |
| Gene ID | 958 |
| Full Name | CD40 molecule |
| Alias | Bp50, CDW40, TNFRSF5, p50 |
| Introduction | CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules. |
| Product Details | |
|---|---|
| Host | Human |
| Clonality | Monoclonal |
| Clone No. | 5D12 |
| Isotype | IgG4 Kappa |
| Immunogen | Anti-CD40/TNFRSF5 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40/TNFRSF5 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12. |
| Usage | |
|---|---|
| Application | ELISA, FC, IHC, IP, SPR |
| Reactivity | Human, Primate-Cynomolgus Monkey |
| Specificity | ch5D12 binds specifically to recombinant and native human CD40/TNFRSF5 ectodomain and has been shown to also bind CD40/TNFRSF5 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40/TNFRSF5 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40/TNFRSF5 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.02% Proclin 300 |
| Storage Temp. | Store at 4 °C for up to 3 months. For longer storage, aliquot and store at -20 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.