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CD25/IL-2R alpha Antibody
SPA-05741
| Size | Price |
| 0.5 mL | Online Inquiry |
| 0.1 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-2 Receptor |
| Gene Abbr. | IL2RA |
| Gene ID | 3559 |
| Full Name | interleukin 2 receptor subunit alpha |
| Alias | CD25, IDDM10, IL2R, IMD41, TCGFR |
| Introduction | The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 22722 |
| Isotype | IgG1 |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha. Glu22-Cys213. Accession # P01589. |
| Usage | |
|---|---|
| Application | WB, IHC, IF |
| Dilutions | Western Blot (1 µg/mL); Immunohistochemistry (8-25 µg/mL); Immunofluorescence (8-25 µg/mL); Neutralization (0.5-1.0 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human CD25/IL-2 R alpha in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.