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CCL22/MDC Antibody
SPA-01615
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CCL22/MDC |
| Gene Abbr. | CCL22 |
| Gene ID | 6367 |
| Full Name | C-C motif chemokine ligand 22 |
| Alias | A-152E5.1, ABCD-1, DC/B-CK, MDC, SCYA22 |
| Introduction | Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells. Schaniel, C. et al. (1998) J. Exp. Med. 188:451. Imai, T. et al. (1998) J. Biol. Chem. 273:1764. Godiska, R. et al. (1997) J. Exp. Med. 185:1595. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229. |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | 158132 |
| Isotype | IgG2A |
| Immunogen | E. coli-derived recombinant mouse CCL22/MDC. Gly25-Ser92. O88430. |
| Usage | |
|---|---|
| Application | ELISA |
| Dilutions | ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL); Neutralization (0.4-2.0 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse CCL22/MDC in ELISAs and Western Blots. In Western Blots, this antibody shows 25% cross-reactivity with recombinant viral MIP‑II and no cross-reactivity with rmCCL1, 2, 3, 4, 6, 7, CCL9/10/MIP-1 gamma, 11, 12, 19, 20, 21, 22, 24, 25, 27, rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 28, or rrCCL20. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.