Online Inquiry
Caspase-9 Antibody
SPA-01447
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Caspase-9 |
| Gene Abbr. | CASP9 |
| Gene ID | 842 |
| Full Name | caspase 9 |
| Alias | APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 |
| Introduction | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Human Caspase 9 recombinant protein catalytic subunits (NP_001220). |
| Usage | |
|---|---|
| Application | WB, IF, IHC, IP |
| Dilutions | Immunoprecipitation (1:50-1:200) |
| MW(KDa) | 47/37/35 (H), 49/39/37 (M) |
| Reactivity | Human, Mouse, Rat, Canine, Mammal |
| Specificity | This antibody detects both the pro- and active-Caspase 9 forms. In Western Blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Whole antisera. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.