Calm1 cDNA ORF Clone, Rat, C-His tag - CD BioSciences

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Calm1 cDNA ORF Clone, Rat, C-His tag

Calm1 cDNA ORF Clone, Rat, C-His tag

SPD-01957

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Description
Full length Clone DNA of Rat calmodulin 1 with C terminal His tag.
Target Information
Species Rat
Target Name Calmodulin
Gene Abbr. Calm1
Gene ID 24244
Full Name calmodulin 2
Alias CaMI, Calm, Cam1
Introduction Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement. Upon binding of four Ca2+ ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca2+ pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase. Since calmodulin binds Ca2+ in a cooperative fashion, small changes in cytosolic Ca2+ levels lead to large changes in the level of active calmodulin and its target proteins.
Product Details
Description Full length Clone DNA of Rat calmodulin 1 with C terminal His tag.
NCBI Ref Seq NM_031969.2
RefSeq ORF Size 450 bp
Vector pCMV3-C-His
Promoter Enhanced CMV promoter
Tag Sequence His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.