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CACNB2 Knockout Cell Line
SPL-00761
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 493bp insertion |
| Target Information | |
|---|---|
| Target Name | CACNB2 |
| Gene Abbr. | CACNB2 |
| Gene ID | 783 |
| Full Name | calcium voltage-gated channel auxiliary subunit beta 2 |
| Alias | CAB2, CACNLB2, CAVB2, MYSB |
| Species | Human |
| Genomic Locus | chr10:18401958 |
| Transcript | NM_201593 |
| WT Expression Level | 6.48 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Feb 2013]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 493bp insertion in a coding exon of CACNB2. |
| Description | 493bp insertion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | CTCCAGAGATACATCGGAAT |
| PCR Primer |
Forward: TCAGGAAAGTATAGAACAATCCGGG Reverse: AGGGAAGAAACTAGGCTCCATTTTT |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.