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c-Myc Antibody
SPA-01239
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | c-Myc |
| Gene Abbr. | MYC |
| Gene ID | 4609 |
| Full Name | MYC proto-oncogene, bHLH transcription factor |
| Alias | MRTL, MYCC, bHLHe39, c-Myc |
| Introduction | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis. These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription. Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families. The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior. The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 9E10 |
| Isotype | IgG1 Kappa |
| Immunogen | A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]. |
| Usage | |
|---|---|
| Application | WB, ChIP, ELISA, IF, IHC, IP |
| Dilutions | Western Blot (0.5-2.0 µg/mL); ELISA (1:100-1:2000); Flow Cytometry (1:50-1:200); Immunofluorescence (1:50-1:200); Immunohistochemistry (1:50-1:200) |
| MW(KDa) | 62 |
| Reactivity | Human, Mouse, D. melanogaster |
| Specificity | Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.