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c-Kit Antibody
SPA-01805
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CD117/c-kit |
| Gene Abbr. | KIT |
| Gene ID | 3815 |
| Full Name | KIT proto-oncogene, receptor tyrosine kinase |
| Alias | C-Kit, CD117, MASTC, PBT, SCFR |
| Introduction | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | C117/370+KIT/982+KIT/983 |
| Isotype | IgG1 Kappa/IgG1 Kappa/IgG1 Kappa |
| Immunogen | Recombinant human CD117 proteins. |
| Usage | |
|---|---|
| Application | FC, IHC, IF |
| Dilutions | Flow Cytometry (0.5-1 µg/10^6 cells in 0.1 mL); Immunofluorescence (0.5-1.0 µg/mL) |
| Reactivity | Human |
| Specificity | This MAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 10 mM PBS with 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.