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c-IAP1/HIAP-2 Antibody
SPA-01194
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | c-IAP1 |
| Gene Abbr. | BIRC2 |
| Gene ID | 329 |
| Full Name | baculoviral IAP repeat containing 2 |
| Alias | API1, HIAP2, Hiap-2, MIHB, RNF48 |
| Introduction | The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression. In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9. In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to amino acids 2-18 (HKTASQRLFPGPSYQNI) of human cIAP1 was used as immunogen, GenBank no. gi|2497238. |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| Dilutions | Western Blot (1:1000-1:2000); Immunoprecipitation (1:50-1:200) |
| MW(KDa) | 62 |
| Reactivity | Human |
| Specificity | cIAP1 has 618 amino acids and migrates at ~70 kDa on SDS-PAGE. Observed molecular weights may vary as genes generally produce, by alternative splicing, multiple transcripts all with introns, putatively encoding multiple different protein isoforms. Additionally, proteins may undergo post translational modifications including phosphorylation, glycosylation and cleavage which can result in differential molecular weights. Protein modifications can vary between cell and tissue type, and proteins can migrate as one or multiple bands depending on the isoform expressed as well as post-translational modifications. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Whole antisera. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.