Bcl2l1 cDNA ORF Clone, Mouse, C-FLAG tag - CD BioSciences

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Bcl2l1 cDNA ORF Clone, Mouse, C-FLAG tag

Bcl2l1 cDNA ORF Clone, Mouse, C-FLAG tag

SPD-01549

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Description
Full length Clone DNA of Mouse BCL2-like 1 with C terminal Flag tag.
Target Information
Species Mouse
Target Name Bcl-xL
Gene Abbr. Bcl2l1
Gene ID 12048
Full Name BCL2-like 1
Alias Bcl, Bcl(X, Bcl(X)L, Bcl-XL, Bcl2l
Introduction Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions. Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole.
Product Details
Description Full length Clone DNA of Mouse BCL2-like 1 with C terminal Flag tag.
NCBI Ref Seq NM_009743.4
RefSeq ORF Size 741 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-C-FLAG
Promoter Enhanced CMV promoter
Tag Sequence FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Restriction Sites KpnI (two restrictionsites) + XbaI (6kb + 0.3kb + 0.45kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.