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Bcl-6 Antibody
SPA-00972
| Size | Price |
| 100 µL | Online Inquiry |
| 25 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Bcl-6 |
| Gene Abbr. | BCL6 |
| Gene ID | 604 |
| Full Name | BCL6 transcription repressor |
| Alias | BCL5, BCL6A, LAZ3, ZBTB27, ZNF51 |
| Introduction | Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkin's lymphoma. The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors. BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes. Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative. The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells. In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | BCL6/1527 |
| Isotype | IgG1 Kappa |
| Immunogen | Recombinant human bcl-6 protein fragment (around aa256-389) (Exact sequence is proprietary). |
| Usage | |
|---|---|
| Application | FC, IHC |
| Dilutions | Flow Cytometry (0.5-1 μg/10^6 cells) |
| Reactivity | Human |
| Specificity | The specificity of this monoclonal antibody to human Bcl-6 was validated by HuProt Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 10 mM PBS with 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.