Online Inquiry
Bcl-2 Antibody
SPA-00964
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Bcl-2 |
| Gene Abbr. | BCL2 |
| Gene ID | 596 |
| Full Name | BCL2 apoptosis regulator |
| Alias | Bcl-2, PPP1R50 |
| Introduction | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 625509 |
| Isotype | IgG2B |
| Immunogen | E. coli-derived recombinant human Bcl-2. Ala2-Asp211. P10415. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (0.1 µg/mL); Immunohistochemistry (8-25 µg/mL) |
| MW(KDa) | 26 |
| Reactivity | Human, Mouse, Rat |
| Validation | Knockout Validated. |
| Specificity | Detects human Bcl-2 in direct ELISAs and Western Blots. In direct ELISAs, approximately 25‑40% cross‑reactivity with recombinant mouse (rm) Bcl‑2 is observed and no cross-reactivity with recombinant human BCL2L12 is observed. In Western Blots, 100% cross-reactivity with rmBcl-2 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.