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Bcl-2 Antibody
SPA-00952
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Bcl-2 |
| Gene Abbr. | BCL2 |
| Gene ID | 596 |
| Full Name | BCL2 apoptosis regulator |
| Alias | Bcl-2, PPP1R50 |
| Introduction | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to amino acids 61-76 (ASRDPVARTSPLQTPAC) of human Bcl-2 was used as immunogen; GenBank no NP_000648.2. This sequence is 100% conserved in human Bcl-2 alpha (239 amino acids) and beta (205 amino acids) isoforms. The literature indicates that the immunogen sequence is not conserved between human Bcl-2 and other species. Therefore this antibody may be particularly useful for detecting human Bcl-2 in transgenic studies. |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| Dilutions | Western Blot (1:1000-1:2000); Immunoprecipitation (1:50-1:200) |
| MW(KDa) | 26 |
| Reactivity | Human |
| Specificity | Bcl-2. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Whole antisera. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.