ATP9B Knockout Cell Line - CD BioSciences

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ATP9B Knockout Cell Line

ATP9B Knockout Cell Line

SPL-00571

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1 Unit Online Inquiry
Description
95bp deletion
Target Information
Target Name ATP9B
Gene Abbr. ATP9B
Gene ID 374868
Full Name ATPase phospholipid transporting 9B (putative)
Alias ATPASEP, ATPIIB, HUSSY-20, NEO1L, hMMR1
Species Human
Genomic Locus chr18:79096485
Transcript NM_198531
WT Expression Level 40.01 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 95bp deletion in a coding exon of ATP9B.
Description 95bp deletion
Parental Cell Line C631
Guide RNA Sequence GGAGGATGAGTCTGCGCATT
PCR Primer Forward: TAACAGAGACTTCTTCAGCTAGCAC
Reverse: TAACAAACCTGGTACAGAGGAACTT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.