ATP5MG Knockout Cell Line - CD BioSciences

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ATP5MG Knockout Cell Line

ATP5MG Knockout Cell Line

SPL-00566

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Description
1bp insertion
Target Information
Target Name ATP5MG
Gene Abbr. ATP5MG
Gene ID 10632
Full Name ATP synthase membrane subunit g
Alias ATP5JG, ATP5L
Species Human
Genomic Locus chr11:118401674
Transcript NM_006476
WT Expression Level 171.02 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 1bp insertion in a coding exon of ATP5L.
Description 1bp insertion
Parental Cell Line C631
Guide RNA Sequence CCACAAGGTTACGGACAAAT
PCR Primer Forward: GACATTTAAGTATCTTCGACCGGC
Reverse: TAGGTTGTGTAGAGAACCCAATCTG
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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