Online Inquiry
ATG7 (cKO+/-) Cell Line
SPL-00550
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| ATG7 (cKO+/-) |
| Target Information | |
|---|---|
| Target Name | Atg7 |
| Gene Abbr. | ATG7 |
| Gene ID | 10533 |
| Full Name | autophagy related 7 |
| Alias | APG7-LIKE, APG7L, GSA7 |
| Species | Human |
| Genomic Locus | 3p25.3 |
| Introduction | This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]. |
| Product Details | |
|---|---|
| Cell Line Model | DLD-1 |
| Genotype | ATG7 (cKO+/-) |
| Description | ATG7 (cKO+/-) |
| Parental ATCC ID | CCL-221 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 8,11; D16S539: 12,13; D5S818: 13; D7S820: 10,12; THO1: 7,9.3; TPOX: 8,11; vWA: 18,19; ATCC |
| Disease | Colorectal adenocarcinoma, Dukes Type C |
| Tissue | Colon |
| Gender | Male |
| Key Endogenous Mutations | APC, BRCA2, FAM123B, KRAS, MSH6, PI3KA, TP53, |
| CVT Selection Condition | G418 resistant - 0.35 mg/mL; Hygromycin resistant - 0.2 mg/mL |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:10. |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:10. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.