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Atg5 Antibody
SPA-00712
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Atg5 |
| Gene Abbr. | ATG5 |
| Gene ID | 9474 |
| Full Name | autophagy related 5 |
| Alias | APG5, APG5-LIKE, APG5L, ASP, SCAR25 |
| Introduction | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | This ATG5 Antibody was made to a synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]. |
| Usage | |
|---|---|
| Application | WB, ELISA, FC, IF, IHC |
| Dilutions | Western Blot (1:500); Immunofluorescence (1:250); Immunohistochemistry (1:400); Immunoprecipitation (1:10-1:500) |
| MW(KDa) | 55 |
| Reactivity | Human, Mouse, Rat, Porcine, Bovine, Fish, Guinea Pig, Primate, Xenopus, Zebrafish |
| Validation | Knockdown Validated. |
| Specificity | This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.