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Atg4B Antibody
SPA-00692
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Atg4B |
| Gene Abbr. | ATG4B |
| Gene ID | 23192 |
| Full Name | autophagy related 4B cysteine peptidase |
| Alias | APG4B, AUTL1 |
| Introduction | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog.Phosphorylation of Atg4B by MST4 increases its activity and autophagic flux. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 515803 |
| Isotype | IgG2B |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4B/Apg4b. Asp2-Leu393 (predicted). Accession # Q9Y4P1. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1 µg/mL); Immunoprecipitation (25 µg/mL) |
| MW(KDa) | 48 |
| Reactivity | Human |
| Specificity | Detects human ATG4B/Apg4b in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, no cross‑reactivity with recombinant human ATG4A is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.