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Asymmetric-Methyl-PABP1 (Arg455/Arg460) Antibody
SPA-08571
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PABP1 |
| Gene Abbr. | PABPC1 |
| Gene ID | 26986 |
| Full Name | poly(A) binding protein cytoplasmic 1 |
| Alias | PAB1, PABP, PABP1, PABPC2, PABPL1 |
| Introduction | Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F. eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover. Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm. PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase; however, the function of this methylation has yet to be determined. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C60A10 |
| Immunogen | Synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated. |
| Usage | |
|---|---|
| Application | WB, IP, IF, IHC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:25) |
| MW(KDa) | 71 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.