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ARID1A Knockout Cell Line
SPL-00399
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 154bp insertion |
| Target Information | |
|---|---|
| Target Name | ARID1A |
| Gene Abbr. | ARID1A |
| Gene ID | 8289 |
| Full Name | AT-rich interaction domain 1A |
| Alias | B120, BAF250, BAF250a, BM029, C1orf4 |
| Species | Human |
| Genomic Locus | chr1:26729696 |
| Transcript | NM_006015 |
| WT Expression Level | 32.74 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 154bp insertion in a coding exon of ARID1A. |
| Description | 154bp insertion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | TGGGTTAGTCCCGCCATA |
| PCR Primer |
Forward: TACTAGGTTGGTCTCATTGCTCTTT Reverse: GGTCAAGGTAATCACAATCACCATC |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.