ARHGAP32 Knockout Cell Line - CD BioSciences

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ARHGAP32 Knockout Cell Line

ARHGAP32 Knockout Cell Line

SPL-00351

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1 Unit Online Inquiry
Description
17bp deletion
Target Information
Target Name ARHGAP32
Gene Abbr. ARHGAP32
Gene ID 9743
Full Name Rho GTPase activating protein 32
Alias GC-GAP, GRIT, PX-RICS, RICS, p200RhoGAP
Species Human
Genomic Locus chr11:128986620
Transcript NM_001142685
WT Expression Level 3.98 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 17bp deletion in a coding exon of ARHGAP32.
Description 17bp deletion
Parental Cell Line C631
Guide RNA Sequence ATACGGTTCTTTCGTCAGGT
PCR Primer Forward: TTTGAACAAAAGCACAGCAAAGTTC
Reverse: TACTAAGACATCCAGTTGTTGCTGA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.