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APAF1 cDNA ORF Clone, Human, In cloning vector
SPD-00709
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| Full length Clone DNA of Human apoptotic peptidase activating factor 1 |
| Target Information | |
|---|---|
| Species | Human |
| Target Name | Apaf-1 |
| Gene Abbr. | APAF1 |
| Gene ID | 317 |
| Full Name | apoptotic peptidase activating factor 1 |
| Alias | APAF-1, CED4 |
| Introduction | Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat. Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli. |
| Product Details | |
|---|---|
| Description | Full length Clone DNA of Human apoptotic peptidase activating factor 1 |
| NCBI Ref Seq | NM_181868.1 |
| RefSeq ORF Size | 3618 bp |
| Sequence Information | Identical with the Gene Bank Ref. ID sequence. |
| Vector | pGEM-T Vector |
| Quality Control | The plasmid is confirmed by full-length sequencing. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
| Usage | |
|---|---|
| Sequencing Primers | SP6 and T7 or M13-47 and RV-M |
| Antibiotic in E.coli | Ampicillin |
| Shipping | Each tube contains lyophilized plasmid. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.