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Apaf-1 Antibody
SPA-00534
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Apaf-1 |
| Gene Abbr. | APAF1 |
| Gene ID | 317 |
| Full Name | apoptotic peptidase activating factor 1 |
| Alias | APAF-1, CED4 |
| Introduction | Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat. Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D5C3 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Pro470 of human Apaf-1 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro470 of human Apaf-1 protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 135 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total Apaf-1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.