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alpha 2-Macroglobulin Antibody
SPA-00416
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | alpha 2-Macroglobulin |
| Gene Abbr. | A2M |
| Gene ID | 2 |
| Full Name | alpha-2-macroglobulin |
| Alias | A2MD, CPAMD5, FWP007, S863-7 |
| Introduction | Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5. alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Human plasma-derived alpha 2-Macroglobulin. |
| Usage | |
|---|---|
| Application | WB, IP, IF |
| Dilutions | Western Blot (0.5 µg/mL); Immunoprecipitation (25 µg/mL); Immunofluorescence (5-15 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human alpha 2-Macroglobulin in direct ELISAs and Western Blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha 2-Macroglobulin-like 1 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.