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AKT1S1 Knockout Cell Line
SPL-00192
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 56bp deletion |
| Target Information | |
|---|---|
| Target Name | AKT1S1 |
| Gene Abbr. | AKT1S1 |
| Gene ID | 84335 |
| Full Name | AKT1 substrate 1 |
| Alias | Lobe, PRAS40 |
| Species | Human |
| Genomic Locus | chr19:49873220 |
| Transcript | NM_032375 |
| WT Expression Level | 36.83 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 56bp deletion in a coding exon of AKT1S1. |
| Description | 56bp deletion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | CTTCCGGGCCCGGACTGGCA |
| PCR Primer |
Forward: TCTCACCTCCATTATCACTAATGCC Reverse: AAAGGGGAAGGAGTACAGTGACTT |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.