Online Inquiry
ADAMTS3 Antibody
SPA-00219
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ADAMTS3 |
| Gene Abbr. | ADAMTS3 |
| Gene ID | 9508 |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif 3 |
| Alias | ADAMTS-4, HKLLS3 |
| Introduction | ADAMTS3 (A disintegrin and metalloprotease with thrombospondin motifs 3; also PC II-NP) is a 140-150 kDa member of the ADAMTS family of Zn metalloproteases. It is expressed by osteoblasts, chrondrocytes, myoepithelium and fibroblasts, and participates in collagen maturation. Together with ADAMTS2 and TS14, ADAMTS3 is known to process the N-terminus of procollagen. In particular, it cleaves the N-terminal globular region of collagen I and II, leading to fibril formation. Mature human proADAMTS3 is a secreted, 1185 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 38-201), a peptidase M12B domain (aa 256-460), a disintegrin region (aa 470-550), four TSP type I sequences (aa 551-1015), and a PLAC domain (aa 1015-1054). There are two potential splice variants. One shows a 38 aa substitution for aa 169-1205, while another shows an alternative start site at Met171. Over aa 19-712, human ADAMTS3 shares 91% aa identity with mouse ADAMTS3. |
| Product Details | |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS3. Ser19-Cys712. Accession # O15072. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (0.1 µg/mL); Immunoprecipitation (25 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human ADAMTS3 in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, approximately 3% cross-reactivity with recombinant human (rh) ADAMTS4 and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS5, rhADAMTS13, and rhADAMTSL1.2 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.