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ADAMTS13 Antibody
SPA-00210
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ADAMTS13 |
| Gene Abbr. | ADAMTS13 |
| Gene ID | 11093 |
| Full Name | ADAM metallopeptidase with thrombospondin type 1 motif 13 |
| Alias | ADAM-TS13, ADAMTS-13, C9orf8, VWFCP, vWF-CP |
| Introduction | ADAMTS13 was first identified by its ability to cleave the ultra large aggregates of vWF into smaller forms in the serum. A thrombocytopenic disorder called thrombic thrombocytopenic purpura (TTR) was shown to be due to a deficiency in the cleavage of von Willebrands factor, and ADAMTS13 was identified as the enzyme responsible. ADAMTS13 cleaves the Tyr1605 to Met1606 bond in the A2 domain of vWF, a process stimulated by shear stress in the circulation and by binding to either platelet glycoprotein IBa or to heparin. The cleaved vWF leads to decreased platelet adhesion. An idiopathic form of TTR is found in patients with auto antibodies to ADAMTS13. The full length ADAMTS-1 sequence codes for a 1,427 amino acid protein, with a predicted mass of 153.6 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels. The variants reported include 1,371, 1,340 and 344 amino acids, with predicted mass of 130.3, 144.5 and 36.7 kDa respectively. Many bands of varying sizes can be seen on Western blots, between 110-190 kDa, perhaps indicating differentially processed ADAMTS-13. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 442323 |
| Isotype | IgG1 |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13 isoform 1. Gln34-Trp688 (predicted). Accession # Q76LX8. |
| Usage | |
|---|---|
| Application | WB, IP, FC |
| Dilutions | Western Blot (1 µg/mL); Immunoprecipitation (25 µg/mL); Flow Cytometry (2.5 µg/10^6 cells) |
| Reactivity | Human |
| Specificity | Detects human ADAMTS13 in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, no cross-reactivity with recombinant human (rh) ADAMTS1, rhADAMTS4, rhADAMTS5, or rhADAMTS-L1.2 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.