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Acinus Antibody
SPA-00139
| Size | Price |
| 0.05 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Acinus |
| Gene Abbr. | ACIN1 |
| Gene ID | 22985 |
| Full Name | apoptotic chromatin condensation inducer 1 |
| Alias | ACINUS, ACN, fSAP152 |
| Introduction | Acinus (apoptotic chromatin condensation inducer in the nucleus) is a caspase substrate that has been implicated in nuclear changes during apoptosis. Chromatin condensation and DNA fragmentation are both nuclear morphological features associated with apoptosis. Acinus is expressed in different isoforms (L, S, S') most likely generated by alternative splicing. During apoptosis Acinus is cleaved by caspase-3 to generate a 23 kDa fragment that was reported to induce chromatin condensation. Acinus has been identified to be a component of the spliceosome complex, ASAP, suggesting a role in pre-mRNA processing. Down regulation of Acinus by RNA interference inhibits cell growth. This study also found that loss of Acinus inhibits DNA fragmentation but not chromatin condensation during apoptosis. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ala835 of Acinus. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala835 of Acinus. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 220, 84, 86 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of total Acinus protein. The antibody recognizes L, S and S' isoforms. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.