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Acetyl-Histone H3 (Lys14/Lys9) Antibody
SPA-05151
| Size | Price |
| 50 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Histone H3 |
| Gene Abbr. | H3C14 |
| Gene ID | 126961 |
| Full Name | H3 clustered histone 14 |
| Alias | H3, H3.2, H3/M, H3C13, H3C15 |
| Introduction | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | This Histone H3 [ac Lys9, ac Lys14] antibody was raised against synthetic acetylated peptide surrounding Lysines 9 and 14 of human Histone H3.2 [Swiss Prot Q71DI3]. |
| Usage | |
|---|---|
| Application | WB, ChIP, FC, IF |
| Dilutions | Western Blot (1:500); Chromatin Immunoprecipitation (2-5 µg/10^6 cells); Dot Blot (0.1 µg/mL); Immunofluorescence (1:200) |
| MW(KDa) | 17 |
| Reactivity | Human, Mouse, Rat, C. elegans, Rat, Chicken, D. melanogaster, Plant, Xenopus |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS and 30% Glycerol. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.