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Acetyl-β-Catenin (Lys49) Antibody
SPA-12006
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | β-Catenin |
| Gene Abbr. | CTNNB1 |
| Gene ID | 1499 |
| Full Name | catenin beta 1 |
| Alias | CTNNB, EVR7, MRD19, NEDSDV, armadillo |
| Introduction | β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines.Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma. CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D7C2 |
| Immunogen | Synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:200) |
| MW(KDa) | 92 |
| Reactivity | Human, Mouse, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of β-catenin protein only when acetylated at Lys49. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.